Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Clinical immunology (Orlando, Fla.)

Article Title: Migration of regulatory T cells toward airway epithelial cells is impaired in chronic rhinosinusitis with nasal polyposis

doi: 10.1016/j.clim.2010.05.013

Figure Lengend Snippet: Chemotactic patterns of Treg (A and D) and non-Treg (B and E) cells toward bronchial epithelial cells (BECs, A, B and C) and primary nasal epithelial cells (PNECs, D and E). Treg: non-Treg migration indices toward BECs using the same sample are shown (C).

Article Snippet: Human BECs (PromoCell, Heidelberg, Germany) were purchased and maintained in Airway Epithelial Cell growth medium (Airway Medium-2, PromoCell, Heidelberg, Germany) supplemented with 50 U–50 mg/ml of penicillin–streptomycin (Gibco, Carlsbad, CA) in a humidified atmosphere containing 5% CO 2 at 37 C. Cells were seeded into 6-well culture plates (BD Biosciences, San Jose, CA) at a density of 5×10 5 cells/well.

Techniques: Migration